Signalling Nanodomains Lab Visualising the structures of intracellular communication, one molecule at a time

Publications

Our peer-reviewed publications include papers on (i.) calcium signalling nanodomains in health and disease, (ii.) novel imaging tools and protocols for improving the usability, versatility, and accessibility of superresolution microscopy, and (iii.) matters arising on Equality, Diversity, Inclusion & Accessibility in academic STEM workplaces.

We are signatories to the San Francisco Declaration on Research Assessment. We publish only research of the highest quality and rigour, given the resources available to us and circumstances that we work within. Our papers are published in journals that are most appropriate for the primary readership of this research. The journals reputations, the rankings, and citation metrics of our publications are of little relevance to us.

Highlighted

2024

Super-Resolution Analysis of the Origins of the Elementary Events of ER Calcium Release in Dorsal Root Ganglion Neurons
Super-Resolution Analysis of the Origins of the Elementary Events of ER Calcium Release in Dorsal Root Ganglion Neurons
Miriam E. Hurley, Shihab S. Shah, Thomas M. D. Sheard, Hannah M. Kirton, Derek S. Steele, Nikita Gamper, Izzy Jayasinghe
Cells   ·   03 Jan 2024   ·   doi:10.3390/cells13010038
Coordinated events of calcium (Ca2+) released from the endoplasmic reticulum (ER) are key second messengers in excitable cells. In pain-sensing dorsal root ganglion (DRG) neurons, these events can be observed as Ca2+ sparks, produced by a combination of ryanodine receptors (RyR) and inositol 1,4,5-triphosphate receptors (IP3R1). These microscopic signals offer the neuronal cells with a possible means of modulating the subplasmalemmal Ca2+ handling, initiating vesicular exocytosis. With super-resolution dSTORM and expansion microscopies, we visualised the nanoscale distributions of both RyR and IP3R1 that featured loosely organised clusters in the subplasmalemmal regions of cultured rat DRG somata. We adapted a novel correlative microscopy protocol to examine the nanoscale patterns of RyR and IP3R1 in the locality of each Ca2+ spark. We found that most subplasmalemmal sparks correlated with relatively small groups of RyR whilst larger sparks were often associated with larger groups of IP3R1. These data also showed spontaneous Ca2+ sparks in <30% of the subplasmalemmal cell area but consisted of both these channel species at a 3.8–5 times higher density than in nonactive regions of the cell. Taken together, these observations reveal distinct patterns and length scales of RyR and IP3R1 co-clustering at contact sites between the ER and the surface plasmalemma that encode the positions and the quantity of Ca2+ released at each Ca2+ spark.

2023

Geometry-preserving expansion microscopy microplates enable high-fidelity nanoscale distortion mapping
Geometry-preserving expansion microscopy microplates enable high-fidelity nanoscale distortion mapping
Rajpinder S. Seehra, Samantha J. Warrington, Benjamin H.K. Allouis, Thomas M.D. Sheard, Michael E. Spencer, Tayla Shakespeare, Ashley Cadby, Daniel Bose, David Strutt, Izzy Jayasinghe
Cell Reports Physical Science   ·   01 Dec 2023   ·   doi:10.1016/j.xcrp.2023.101719
Expansion microscopy (ExM) is a versatile super-resolution microscopy pipeline, leveraging nanoscale biomolecular crosslinking and osmotically driven swelling of hydrogels. Currently, ExM is a laborious and skill-intensive technique, involving manual handling of the hydrogels that can compromise the integrity of the gels and capacity to track gel isotropy, hence diminishing reproducibility. We have developed a 3D-printable microplate system to contain the entire ExM workflow within each well, enabling in situ image acquisition and eliminating the need for direct handling of the hydrogels. The preservation of the gel geometry and orientation of the microplate wells enables convenient tracking of gel expansion, pre- and post-ExM image acquisition, and distortion mapping of every cell or region of interest. We demonstrate the utility of this approach with both single-color and multiplexed ExM of cultured HeLa cells and dissected pupal Drosophila melanogaster wing tissue to reveal distortion-prone structures ranging from sub-cellular organelles to micron-scale tissue regions.
Correlative super-resolution analysis of cardiac calcium sparks and their molecular origins in health and disease
Correlative super-resolution analysis of cardiac calcium sparks and their molecular origins in health and disease
Miriam E. Hurley, Ed White, Thomas M. D. Sheard, Derek Steele, Izzy Jayasinghe
Open Biology   ·   24 May 2023   ·   doi:10.1098/rsob.230045
Rapid release of calcium from internal stores via ryanodine receptors (RyRs) is one of the fastest types of cytoplasmic second messenger signalling in excitable cells. In the heart, rapid summation of the elementary events of calcium release, ‘calcium sparks’, determine the contraction of the myocardium. We adapted a correlative super-resolution microscopy protocol to correlate sub-plasmalemmal spontaneous calcium sparks in rat right ventricular myocytes with the local nanoscale RyR2 positions. This revealed a steep relationship between the integral of a calcium spark and the sum of the local RyR2s. Segmentation of recurring spark sites showed evidence of repeated and triggered saltatory activation of multiple local RyR2 clusters. In myocytes taken from failing right ventricles, RyR2 clusters themselves showed a dissipated morphology and fragmented (smaller) clusters. They also featured greater heterogeneity in both the spark properties and the relationship between the integral of the calcium spark and the local ensemble of RyR2s. While fragmented (smaller) RyR2 clusters were rarely observed directly underlying the larger sparks or the recurring spark sites, local interrogation of the channel-to-channel distances confirmed a clear link between the positions of each calcium spark and the tight, non-random clustering of the local RyR2 in both healthy and failing ventricles.

All papers

2024

Cysteine post‐translational modifications regulate protein interactions of caveolin‐3
Cysteine post‐translational modifications regulate protein interactions of caveolin‐3
Fiona Ashford, Chien‐Wen Kuo, Emma Dunning, Elaine Brown, Sarah Calagan, Izzy Jayasinghe, Colin Henderson, William Fuller, Krzysztof Wypijewski
The FASEB Journal   ·   11 Mar 2024   ·   doi:10.1096/fj.202201497RR
Super-Resolution Analysis of the Origins of the Elementary Events of ER Calcium Release in Dorsal Root Ganglion Neurons
Super-Resolution Analysis of the Origins of the Elementary Events of ER Calcium Release in Dorsal Root Ganglion Neurons
Miriam E. Hurley, Shihab S. Shah, Thomas M. D. Sheard, Hannah M. Kirton, Derek S. Steele, Nikita Gamper, Izzy Jayasinghe
Cells   ·   03 Jan 2024   ·   doi:10.3390/cells13010038
Coordinated events of calcium (Ca2+) released from the endoplasmic reticulum (ER) are key second messengers in excitable cells. In pain-sensing dorsal root ganglion (DRG) neurons, these events can be observed as Ca2+ sparks, produced by a combination of ryanodine receptors (RyR) and inositol 1,4,5-triphosphate receptors (IP3R1). These microscopic signals offer the neuronal cells with a possible means of modulating the subplasmalemmal Ca2+ handling, initiating vesicular exocytosis. With super-resolution dSTORM and expansion microscopies, we visualised the nanoscale distributions of both RyR and IP3R1 that featured loosely organised clusters in the subplasmalemmal regions of cultured rat DRG somata. We adapted a novel correlative microscopy protocol to examine the nanoscale patterns of RyR and IP3R1 in the locality of each Ca2+ spark. We found that most subplasmalemmal sparks correlated with relatively small groups of RyR whilst larger sparks were often associated with larger groups of IP3R1. These data also showed spontaneous Ca2+ sparks in <30% of the subplasmalemmal cell area but consisted of both these channel species at a 3.8–5 times higher density than in nonactive regions of the cell. Taken together, these observations reveal distinct patterns and length scales of RyR and IP3R1 co-clustering at contact sites between the ER and the surface plasmalemma that encode the positions and the quantity of Ca2+ released at each Ca2+ spark.

2023

Geometry-preserving expansion microscopy microplates enable high-fidelity nanoscale distortion mapping
Geometry-preserving expansion microscopy microplates enable high-fidelity nanoscale distortion mapping
Rajpinder S. Seehra, Samantha J. Warrington, Benjamin H.K. Allouis, Thomas M.D. Sheard, Michael E. Spencer, Tayla Shakespeare, Ashley Cadby, Daniel Bose, David Strutt, Izzy Jayasinghe
Cell Reports Physical Science   ·   01 Dec 2023   ·   doi:10.1016/j.xcrp.2023.101719
Expansion microscopy (ExM) is a versatile super-resolution microscopy pipeline, leveraging nanoscale biomolecular crosslinking and osmotically driven swelling of hydrogels. Currently, ExM is a laborious and skill-intensive technique, involving manual handling of the hydrogels that can compromise the integrity of the gels and capacity to track gel isotropy, hence diminishing reproducibility. We have developed a 3D-printable microplate system to contain the entire ExM workflow within each well, enabling in situ image acquisition and eliminating the need for direct handling of the hydrogels. The preservation of the gel geometry and orientation of the microplate wells enables convenient tracking of gel expansion, pre- and post-ExM image acquisition, and distortion mapping of every cell or region of interest. We demonstrate the utility of this approach with both single-color and multiplexed ExM of cultured HeLa cells and dissected pupal Drosophila melanogaster wing tissue to reveal distortion-prone structures ranging from sub-cellular organelles to micron-scale tissue regions.
4D spatial-spectral super-resolution imaging of nanoscopic membrane signalling domains
4D spatial-spectral super-resolution imaging of nanoscopic membrane signalling domains
[no author info]
Optics InfoBase Conference Papers   ·   09 Oct 2023   ·   eid:2-s2.0-84893627791
Correlative super-resolution analysis of cardiac calcium sparks and their molecular origins in health and disease
Correlative super-resolution analysis of cardiac calcium sparks and their molecular origins in health and disease
Miriam E. Hurley, Ed White, Thomas M. D. Sheard, Derek Steele, Izzy Jayasinghe
Open Biology   ·   24 May 2023   ·   doi:10.1098/rsob.230045
Rapid release of calcium from internal stores via ryanodine receptors (RyRs) is one of the fastest types of cytoplasmic second messenger signalling in excitable cells. In the heart, rapid summation of the elementary events of calcium release, ‘calcium sparks’, determine the contraction of the myocardium. We adapted a correlative super-resolution microscopy protocol to correlate sub-plasmalemmal spontaneous calcium sparks in rat right ventricular myocytes with the local nanoscale RyR2 positions. This revealed a steep relationship between the integral of a calcium spark and the sum of the local RyR2s. Segmentation of recurring spark sites showed evidence of repeated and triggered saltatory activation of multiple local RyR2 clusters. In myocytes taken from failing right ventricles, RyR2 clusters themselves showed a dissipated morphology and fragmented (smaller) clusters. They also featured greater heterogeneity in both the spark properties and the relationship between the integral of the calcium spark and the local ensemble of RyR2s. While fragmented (smaller) RyR2 clusters were rarely observed directly underlying the larger sparks or the recurring spark sites, local interrogation of the channel-to-channel distances confirmed a clear link between the positions of each calcium spark and the tight, non-random clustering of the local RyR2 in both healthy and failing ventricles.
Differential labelling of human sub-cellular compartments with fluorescent dye esters and expansion microscopy
Differential labelling of human sub-cellular compartments with fluorescent dye esters and expansion microscopy
Thomas M. D. Sheard, Tayla Shakespeare, Rajpinder S. Seehra, Michael E Spencer, Kin M. Suen, Izzy Jayasinghe
Cold Spring Harbor Laboratory   ·   22 Feb 2023   ·   doi:10.1101/2023.02.21.529394
Geometry-preserving Expansion Microscopy microplates enable high fidelity nanoscale distortion mapping
Geometry-preserving Expansion Microscopy microplates enable high fidelity nanoscale distortion mapping
Rajpinder S. Seehra, Benjamin H.K. Allouis, Thomas M.D. Sheard, Michael E Spencer, Tayla Shakespeare, Ashley Cadby, Izzy Jayasinghe
Cold Spring Harbor Laboratory   ·   21 Feb 2023   ·   doi:10.1101/2023.02.20.529230
Correlative super-resolution analysis of cardiac calcium sparks and their molecular origins in health and disease
Correlative super-resolution analysis of cardiac calcium sparks and their molecular origins in health and disease
Miriam E. Hurley, Ed White, Thomas M.D. Sheard, Derek Steele, Izzy Jayasinghe
Cold Spring Harbor Laboratory   ·   13 Feb 2023   ·   doi:10.1101/2023.02.12.528168
Expansion microscopy reveals subdomains in<i>C. elegans</i>germ granules
Expansion microscopy reveals subdomains inC. elegansgerm granules
Kin M Suen, Thomas MD Sheard, Chi-Chuan Lin, Dovile Milonaityte, Izzy Jayasinghe, John E Ladbury
Life Science Alliance   ·   07 Feb 2023   ·   doi:10.26508/lsa.202201650
Differential labelling of human sub-cellular compartments with fluorescent dye esters and expansion microscopy
Differential labelling of human sub-cellular compartments with fluorescent dye esters and expansion microscopy
Thomas M. D. Sheard, Tayla B. Shakespeare, Rajpinder S. Seehra, Michael E. Spencer, Kin M. Suen, Izzy Jayasinghe
Nanoscale   ·   01 Jan 2023   ·   doi:10.1039/d3nr01129a

2022

Dismantling barriers faced by women in STEM
Dismantling barriers faced by women in STEM
J. M. Jebsen, K. Nicoll Baines, R. A. Oliver, I. Jayasinghe
Nature Chemistry   ·   27 Oct 2022   ·   doi:10.1038/s41557-022-01072-2
Three-dimensional visualization of the cardiac ryanodine receptor clusters and the molecular-scale fraying of dyads
Three-dimensional visualization of the cardiac ryanodine receptor clusters and the molecular-scale fraying of dyads
Thomas M. D. Sheard, Miriam E. Hurley, Andrew J. Smith, John Colyer, Ed White, Izzy Jayasinghe
Philosophical Transactions of the Royal Society B: Biological Sciences   ·   03 Oct 2022   ·   doi:10.1098/rstb.2021.0316
Increased SERCA2a sub-cellular heterogeneity in right-ventricular heart failure inhibits excitation-contraction coupling and modulates arrhythmogenic dynamics
Increased SERCA2a sub-cellular heterogeneity in right-ventricular heart failure inhibits excitation-contraction coupling and modulates arrhythmogenic dynamics
M. Holmes, M. E. Hurley, T. M. D. Sheard, A. P. Benson, I. Jayasinghe, M. A. Colman
Philosophical Transactions of the Royal Society B: Biological Sciences   ·   03 Oct 2022   ·   doi:10.1098/rstb.2021.0317
Cysteine post-translational modifications regulate protein interactions of caveolin-3
Cysteine post-translational modifications regulate protein interactions of caveolin-3
Fiona Ashford, Elaine Brown, Sarah Calagan, Izzy Jayasinghe, Colin Henderson, William Fuller, Krzysztof Wypijewski
Cold Spring Harbor Laboratory   ·   15 Sep 2022   ·   doi:10.1101/2022.09.15.508083
Expansion microscopy reveals subdomains in <i>C. elegans</i> germ granules
Expansion microscopy reveals subdomains in C. elegans germ granules
Kin M. Suen, Thomas M. D. Sheard, Chi-Chuan Lin, Dovile Milonaityte, Izzy Jayasinghe, John E. Ladbury
Cold Spring Harbor Laboratory   ·   29 May 2022   ·   doi:10.1101/2022.05.29.493872
Conversations with LGBT+ scientists about visibility, leadership and climbing the career ladder
Conversations with LGBT+ scientists about visibility, leadership and climbing the career ladder
Robert G. Bristow, Joanne Engel, Izzy Jayasinghe, Martin Kampmann, Owen James Sansom, David M. Bryant
Journal of Cell Science   ·   15 Feb 2022   ·   doi:10.1242/jcs.259880

2021

Molecular-scale 3D visualisation of the cardiac ryanodine receptor clusters and the molecular-scale fraying of dyads
Molecular-scale 3D visualisation of the cardiac ryanodine receptor clusters and the molecular-scale fraying of dyads
Thomas M. D. Sheard, Miriam E. Hurley, Andrew J. Smith, John Colyer, Ed White, Izzy Jayasinghe
Cold Spring Harbor Laboratory   ·   22 Oct 2021   ·   doi:10.1101/2021.10.21.462365
Multi-scale approaches for the simulation of cardiac electrophysiology: I – Sub-cellular and stochastic calcium dynamics from cell to organ
Multi-scale approaches for the simulation of cardiac electrophysiology: I – Sub-cellular and stochastic calcium dynamics from cell to organ
Michael A. Colman, Maxx Holmes, Dominic G. Whittaker, Izzy Jayasinghe, Alan P. Benson
Methods   ·   01 Jan 2021   ·   doi:10.1016/j.ymeth.2020.02.011

2020

Peptide ligands of the cardiac ryanodine receptor as super-resolution imaging probes
Peptide ligands of the cardiac ryanodine receptor as super-resolution imaging probes
Thomas M. D. Sheard, Luke Howlett, Hannah Kirton, Zhaokang Yang, Georgina Gurrola, Derek Steele, Izzy Jayasinghe, Hector H. Valdivia, John Colyer
Cold Spring Harbor Laboratory   ·   28 Jun 2020   ·   doi:10.1101/2020.06.26.131854
Self-activated photoblinking of nitrogen vacancy centers in nanodiamonds (sandSTORM): A method for rapid single molecule localization microscopy with unlimited observation time
Self-activated photoblinking of nitrogen vacancy centers in nanodiamonds (sandSTORM): A method for rapid single molecule localization microscopy with unlimited observation time
Kaarjel K. Narayanasamy, Joshua C. Price, Raquel Mesquita-Riberio, Melissa L. Mather, Izzy Jayasinghe
Cold Spring Harbor Laboratory   ·   21 May 2020   ·   doi:10.1101/2020.05.20.106716

2019

Rab46 integrates Ca2+ and histamine signaling to regulate selective cargo release from Weibel-Palade bodies
Rab46 integrates Ca2+ and histamine signaling to regulate selective cargo release from Weibel-Palade bodies
Katarina T. Miteva, Lucia Pedicini, Lesley A. Wilson, Izzy Jayasinghe, Raphael G. Slip, ..., Fiona Bartoli, Shruthi Deivasigamani, Diego Sobradillo, David J. Beech, Lynn McKeown
Journal of Cell Biology   ·   15 May 2019   ·   doi:10.1083/jcb.201810118
Three-Dimensional and Chemical Mapping of Intracellular Signaling Nanodomains in Health and Disease with Enhanced Expansion Microscopy
Three-Dimensional and Chemical Mapping of Intracellular Signaling Nanodomains in Health and Disease with Enhanced Expansion Microscopy
Thomas M. D. Sheard, Miriam E. Hurley, John Colyer, Ed White, Ruth Norman, ..., Andrew J. Smith, David Baddeley, Christian Soeller, Michael A. Colman, Izzy Jayasinghe
ACS Nano   ·   08 Feb 2019   ·   doi:10.1021/acsnano.8b08742

2018

Shining New Light on the Structural Determinants of Cardiac Couplon Function: Insights From Ten Years of Nanoscale Microscopy
Shining New Light on the Structural Determinants of Cardiac Couplon Function: Insights From Ten Years of Nanoscale Microscopy
Izzy Jayasinghe, Alexander H. Clowsley, Oscar de Langen, Sonali S. Sali, David J. Crossman, Christian Soeller
Frontiers in Physiology   ·   22 Oct 2018   ·   doi:10.3389/fphys.2018.01472
True Molecular Scale Visualization of Variable Clustering Properties of Ryanodine Receptors
True Molecular Scale Visualization of Variable Clustering Properties of Ryanodine Receptors
Izzy Jayasinghe, Alexander H. Clowsley, Ruisheng Lin, Tobias Lutz, Carl Harrison, Ellen Green, David Baddeley, Lorenzo Di Michele, Christian Soeller
Cell Reports   ·   01 Jan 2018   ·   doi:10.1016/J.CELREP.2017.12.045

2017

Transverse tubule remodelling: a cellular pathology driven by both sides of the plasmalemma?
Transverse tubule remodelling: a cellular pathology driven by both sides of the plasmalemma?
David J. Crossman, Isuru D. Jayasinghe, Christian Soeller
Biophysical Reviews   ·   10 Jul 2017   ·   doi:10.1007/s12551-017-0273-7
Algorithmic corrections for localization microscopy with sCMOS cameras - characterisation of a computationally efficient localization approach
Algorithmic corrections for localization microscopy with sCMOS cameras - characterisation of a computationally efficient localization approach
Ruisheng Lin, Alexander H. Clowsley, Isuru D. Jayasinghe, David Baddeley, Christian Soeller
Optics Express   ·   10 May 2017   ·   doi:10.1364/OE.25.011701
Human skeletal muscle plasmalemma alters its structure to change its Ca2+-handling following heavy-load resistance exercise
Human skeletal muscle plasmalemma alters its structure to change its Ca2+-handling following heavy-load resistance exercise
Tanya R. Cully, Robyn M. Murphy, Llion Roberts, Truls Raastad, Robert G. Fassett, Jeff S. Coombes, Izzy Jayasinghe, Bradley S. Launikonis
Nature Communications   ·   13 Feb 2017   ·   doi:10.1038/ncomms14266
Correlative Single-Molecule Localization Microscopy and Confocal Microscopy
Correlative Single-Molecule Localization Microscopy and Confocal Microscopy
Christian Soeller, Yufeng Hou, Isuru D. Jayasinghe, David Baddeley, David Crossman
Methods in Molecular Biology   ·   01 Jan 2017   ·   doi:10.1007/978-1-4939-7265-4_17

2016

Junctophilin-2 in the nanoscale organisation and functional signalling of ryanodine receptor clusters in cardiomyocytes
Junctophilin-2 in the nanoscale organisation and functional signalling of ryanodine receptor clusters in cardiomyocytes
Michelle L. Munro, Izzy Jayasinghe, Qiongling Wang, Ann Quick, Wei Wang, David Baddeley, Xander H. T. Wehrens, Christian Soeller
Journal of Cell Science   ·   01 Dec 2016   ·   doi:10.1242/jcs.196873

2015

Combining confocal and single molecule localisation microscopy: A correlative approach to multi-scale tissue imaging
Combining confocal and single molecule localisation microscopy: A correlative approach to multi-scale tissue imaging
David J. Crossman, Yufeng Hou, Izzy Jayasinghe, David Baddeley, Christian Soeller
Methods   ·   01 Oct 2015   ·   doi:10.1016/j.ymeth.2015.03.011
Nanoscale analysis of ryanodine receptor clusters in dyadic couplings of rat cardiac myocytes
Nanoscale analysis of ryanodine receptor clusters in dyadic couplings of rat cardiac myocytes
Yufeng Hou, Isuru Jayasinghe, David J. Crossman, David Baddeley, Christian Soeller
Journal of Molecular and Cellular Cardiology   ·   01 Mar 2015   ·   doi:10.1016/j.yjmcc.2014.12.013

2014

Revealing t-tubules in striated muscle with new optical super-resolution microscopy techniques
Revealing t-tubules in striated muscle with new optical super-resolution microscopy techniques
Isuru D. Jayasinghe, Alexander H. Clowsley, Michelle Munro, Yufeng Hou, David J. Crossman, Christian Soeller
European Journal of Translational Myology   ·   24 Dec 2014   ·   doi:10.4081/ejtm.2015.4747
Observation of the molecular organization of calcium release sites in fast- and slow-twitch skeletal muscle with nanoscale imaging
Observation of the molecular organization of calcium release sites in fast- and slow-twitch skeletal muscle with nanoscale imaging
Isuru D. Jayasinghe, Michelle Munro, David Baddeley, Bradley S. Launikonis, Christian Soeller
Journal of The Royal Society Interface   ·   06 Oct 2014   ·   doi:10.1098/rsif.2014.0570
Super-resolution fluorescence imaging to study cardiac biophysics: α-actinin distribution and Z-disk topologies in optically thick cardiac tissue slices
Super-resolution fluorescence imaging to study cardiac biophysics: α-actinin distribution and Z-disk topologies in optically thick cardiac tissue slices
Yufeng Hou, David J. Crossman, Vijay Rajagopal, David Baddeley, Isuru Jayasinghe, Christian Soeller
Progress in Biophysics and Molecular Biology   ·   01 Aug 2014   ·   doi:10.1016/j.pbiomolbio.2014.07.003

2013

Three-dimensional reconstruction and analysis of the tubular system of vertebrate skeletal muscle
Three-dimensional reconstruction and analysis of the tubular system of vertebrate skeletal muscle
Izzy Jayasinghe, Bradley S. Launikonis
Journal of Cell Science   ·   01 Sep 2013   ·   doi:10.1242/jcs.131565
Examination of the Subsarcolemmal Tubular System of Mammalian Skeletal Muscle Fibers
Examination of the Subsarcolemmal Tubular System of Mammalian Skeletal Muscle Fibers
Izzy Jayasinghe, Harriet P. Lo, Garry P. Morgan, David Baddeley, Robert G. Parton, Christian Soeller, Bradley S. Launikonis
Biophysical Journal   ·   01 Jun 2013   ·   doi:10.1016/j.bpj.2013.04.029

2012

Comparison of the organization of t‐tubules, sarcoplasmic reticulum and ryanodine receptors in rat and human ventricular myocardium
Comparison of the organization of t‐tubules, sarcoplasmic reticulum and ryanodine receptors in rat and human ventricular myocardium
ID Jayasinghe, DJ Crossman, C Soeller, MB Cannell
Clinical and Experimental Pharmacology and Physiology   ·   23 Apr 2012   ·   doi:10.1111/j.1440-1681.2011.05578.x
Nanoscale Organization of Junctophilin-2 and Ryanodine Receptors within Peripheral Couplings of Rat Ventricular Cardiomyocytes
Nanoscale Organization of Junctophilin-2 and Ryanodine Receptors within Peripheral Couplings of Rat Ventricular Cardiomyocytes
Izzy Jayasinghe, David Baddeley, Cherrie H.T. Kong, Xander H.T. Wehrens, Mark B. Cannell, Christian Soeller
Biophysical Journal   ·   01 Mar 2012   ·   doi:10.1016/j.bpj.2012.01.034

2011

4D spatial-spectral super-resolution imaging of nanoscopic membrane signalling domains
4D spatial-spectral super-resolution imaging of nanoscopic membrane signalling domains
David Baddeley, Isuru Jayasinghe, Cherrie Kong, David Crossman, Juliette Cheyne, Johanna Montgomery, Mark Cannell, Christian Soeller
2011 International Quantum Electronics Conference (IQEC) and Conference on Lasers and Electro-Optics (CLEO) Pacific Rim incorporating the Australasian Conference on Optics, Lasers and Spectroscopy and the Australian Conference on Optical Fibre Technology   ·   01 Aug 2011   ·   doi:10.1109/IQEC-CLEO.2011.6194007
4D Super-Resolution Microscopy with Conventional Fluorophores and Single Wavelength Excitation in Optically Thick Cells and Tissues
4D Super-Resolution Microscopy with Conventional Fluorophores and Single Wavelength Excitation in Optically Thick Cells and Tissues
David Baddeley, David Crossman, Sabrina Rossberger, Juliette E. Cheyne, Johanna M. Montgomery, Isuru D. Jayasinghe, Christoph Cremer, Mark B. Cannell, Christian Soeller
PLoS ONE   ·   31 May 2011   ·   doi:10.1371/journal.pone.0020645

2010

A new twist in cardiac muscle: dislocated and helicoid arrangements of myofibrillar z-disks in mammalian ventricular myocytes
A new twist in cardiac muscle: dislocated and helicoid arrangements of myofibrillar z-disks in mammalian ventricular myocytes
Isuru D. Jayasinghe, David J. Crossman, Christian Soeller, Mark B. Cannell
Journal of Molecular and Cellular Cardiology   ·   01 May 2010   ·   doi:10.1016/j.yjmcc.2009.12.012

2009

Optical single-channel resolution imaging of the ryanodine receptor distribution in rat cardiac myocytes
Optical single-channel resolution imaging of the ryanodine receptor distribution in rat cardiac myocytes
David Baddeley, Izzy Jayasinghe, Leo Lam, Sabrina Rossberger, Mark B. Cannell, Christian Soeller
Proceedings of the National Academy of Sciences   ·   29 Dec 2009   ·   doi:10.1073/pnas.0908971106
Organization of Ryanodine Receptors, Transverse Tubules, and Sodium-Calcium Exchanger in Rat Myocytes
Organization of Ryanodine Receptors, Transverse Tubules, and Sodium-Calcium Exchanger in Rat Myocytes
Izzy Jayasinghe, Mark B. Cannell, Christian Soeller
Biophysical Journal   ·   01 Nov 2009   ·   doi:10.1016/j.bpj.2009.08.036
Three‐dimensional high‐resolution imaging of cardiac proteins to construct models of intracellular Ca<sup>2+</sup> signalling in rat ventricular myocytes
Three‐dimensional high‐resolution imaging of cardiac proteins to construct models of intracellular Ca2+ signalling in rat ventricular myocytes
Christian Soeller, Isuru D. Jayasinghe, Pan Li, Arun V. Holden, Mark B. Cannell
Experimental Physiology   ·   14 Apr 2009   ·   doi:10.1113/expphysiol.2008.043976
Light-Induced Dark States of Organic Fluochromes Enable 30 nm Resolution Imaging in Standard Media
Light-Induced Dark States of Organic Fluochromes Enable 30 nm Resolution Imaging in Standard Media
David Baddeley, Izzy Jayasinghe, Christoph Cremer, Mark B. Cannell, Christian Soeller
Biophysical Journal   ·   01 Jan 2009   ·   doi:10.1016/j.bpj.2008.11.002